Monocyte differentiation
At BioMedha, we have on-site blood donor panel and collect whole blood from consented healthy donors on the day of experimental setup. Isolated monocytes from fresh healthy donor whole blood were differentiated into dendritic cells and macrophages. Validated by cytokine profiling and surface marker expression suitable for measuring compound effect on cell function and gene expression.
Macrophages
Figure-1: Methods for isolation of monocytes, maintenance, and differentiation into dendritic cells.
Dendritic cells
Figure-2: Methods for isolation of monocytes, maintenance, and differentiation into M1 and M2 phenotype macrophages.
Validation
Figure-3: Cytokine levels (pg/ml) in macrophage supernatant (M1 and M2 phenotype) and surface marker expression levels in polarised macrophages measured by flowcytometry (A). IL-12 (p70) levels (pg/ml) in dendritic cell supernatant and surface marker expression levels in mature dendritic cells measured by flowcytometry (B)
Here we demonstrate successful differentiation of monocytes into dendritic cells and macrophages. Our In vitro assay models enabled us supporting client’s therapeutic programmes, measuring compound effect on dendritic and macrophages cell maturation, polarisation, function, and gene expression.