xCT antiporter assays
xCT (SLC7A11) is a cystine/glutamate antiporter which promotes cystine uptake, glutamate release, and glutathione synthesis. In cancer cells and cancer associated immune cells, SLC7A11 is associated with redox homeostasis, metastasis, metabolism, proliferation, invasion, immune function, and drug resistance.
Cystine Uptake assay
Figure-1: Cystine Uptake against human xCT (A549 cells) and mouse xCT (Raw264.7 cells)
Glutamate release assay
Figure-2: Erastin dose response against Glutamate release assay
Glutathione synthesis assay
Figure-3: Glutathione synthesis in HepG2 cells
At BioMedha, we have established robust xCT (SLC7A11) assays measuring cystine uptake, glutamate release, and glutathione synthesis suitable for profiling compounds. We offer both custom and off-the-shelf assay support. Partner with us to accelerate your drug discovery portfolio.