T cell assays
Robust In vitro assays are curial for characterising drug candidates (biologics or small molecules) modulating immune responses. At BioMedha, we have on-site blood donor panel and collect whole blood from consented healthy donors on the day of experimental setup. We have developed various T cell assays supporting client projects.
T cell activation (Th-1, Th-2 and Th-17 cytokine secretion)
Figure-1: Activation of Th-1, Th-2 and Th-17 cytokine secretion. Freshly isolated human PBMCs from healthy donor were treated with combination of anti-CD3 (plate bound) and anti-CD8 (soluble) for 48 hours before harvesting cell supernatant for cytokine analysis.
T cell proliferation
Figure-2: Fresh PBMCs treated with plate bound anti-CD3 and soluble anti-CD8 for 5 days. Cell proliferation measured by ATPlite reagent.
Tumour cell killing
Figure-3: Cytotoxicity of CD8+ T cells against tumour cells.
CD8 T cell activation
Figure-4: Activation of CD25+ and HLA-DR+ expression in CD8+ T cells.
IL-22 secretion
Figure-5: Freshly isolated T cells from three different healthy donors were treated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator for 72 hours before harvesting supernatant for IL-22 levels analysis.
Phenotyping
Figure-6: T cell phenotyping
T cell exhaustion
Figure-7: Fresh PBMCs treated with Staphylococcal enterotoxin B (SEB) for 72 hours before harvesting supernatant. Cells washed and resuspended into the media and incubated for 24 hours before harvesting supernatant for INFg levels analysis.