GPCR assays
G protein-coupled receptors (GPCRs) considered as one of the most successful therapeutic targets for a broad spectrum of diseases. BioMedha offers GPCR assay support meeting clients project needs include target validation, assay development, hit identification, selectivity profiling, lead optimisation, mechanism of action, and Schild analysis.
Ca 2+ Flux assay
Figure-1: Assay using recombinant cell line.
Figure-2: Assay using transient co-transfection.
Figure-3: Assay using endogenous system.
cAMP assay
Figure-4: DMSO tolerance test on GPR74 cAMP assay readout
IP-one assay
Figure-5: IP-one assay from three independent transient transfections.
pERK-1/2 assay
Figure-6: Measuring C5aR mediated pERK-1/2 levels.
Chemotaxis assay
Figure-7: Inhibition of IL-8 and C5a induced chemotaxis in Neutrophils.
At BioMedha, we have successfully developed various GPCR assays from scratch, supporting client’s project screening cascade. Our GPCR assay cell systems include both recombinant (transient and stable transfections) and endogenous expressing cell systems (e.g. primary immune cells) supporting GPCR assays in multiple assay formats. Partner with us to accelerate your GPCR target portfolio.