xCT antiporter assays
xCT antiporter assays xCT (SLC7A11) is a cystine/glutamate antiporter which promotes cystine uptake, glutamate release, and glutathione synthesis. In cancer cells and cancer associated immune cells, SLC7A11 is associated with redox homeostasis, metastasis, metabolism, proliferation, invasion, immune function, and drug resistance. Cystine Uptake assay Figure-1: Cystine Uptake against human xCT (A549 cells) and mouse xCT […]
Discovery toxicology assays
Discovery toxicology assays Understanding and managing toxicity at the earliest possible is important to improve safety and efficacy of therapeutic molecules in drug development. At BioMedha, we have developed robust tools for implementing and assessing toxicity risk at an early stage. Mitochondrial toxicity Figure-A: Compound dose response curve (4hrs treatment) against Glu/Gal ATP and Mitochondrial […]
Monocyte differentiation
Monocyte differentiation At BioMedha, we have on-site blood donor panel and collect whole blood from consented healthy donors on the day of experimental setup. Isolated monocytes from fresh healthy donor whole blood were differentiated into dendritic cells and macrophages. Validated by cytokine profiling and surface marker expression suitable for measuring compound effect on cell function […]
T cell assays
T cell assays Robust In vitro assays are curial for characterising drug candidates (biologics or small molecules) modulating immune responses. At BioMedha, we have on-site blood donor panel and collect whole blood from consented healthy donors on the day of experimental setup. We have developed various T cell assays supporting client projects. T cell activation […]
B cell assays
B cell assays B cells are important in adaptive immune response and utilisation of activated B cells as drug targets is increasing in immunotherapies. At BioMedha, we have on-site blood donor panel and collect whole blood from consented healthy donors on the day of experimental setup. We have established several B cell activation assays using […]
Whole blood assays
Whole blood assays Whole Blood assays provides a physiological environment and are powerful tools to investigate immune responses. At BioMedha, we have on-site blood donor panel (HTA licenced establishment) and collect whole blood from consented healthy donors on the day of experiment setting up assays. Here, we summarize representative assay readouts developed at BioMedha. CD11b […]
Neutrophil assays
Neutrophil assays Targeting neutrophils is growing in immunology, immuno-oncology, and inflammation therapeutic areas. At BioMedha, we have developed robust In vitro assays using fresh neutrophils isolated from healthy donor whole blood. Suitable for assessing compound effect on neutrophil function. CD11b activation Figure-1: CD11b activation and inhibition in Neutrophils. NETosis Figure-2: PMA induced NETosis in Neutrophils […]
DNA damage assay
DNA damage assay Phosphorylation of histone H2AX on serine139 (pH2AX) is an early and sensitive indicator of DNA double-strand breaks (DSBs) generation and emerged as a highly specific molecular marker for quantifying compound induced DNA damage. Our robust immunofluorescence assay quantifying DNA damage suitable for evaluation of compound effects in throughput format, helps de-risking drug […]