hERG binding assay
hERG binding FP assay Understanding and managing hERG inhibition properties of a compound at the earliest possible is important to improve safety and efficacy of therapeutic molecules. We utilize Predictor hERG binding fluorescence polarization assay as a tool for assessing hERG channel affinity generating IC50 data that tightly correlates with radioligand/ patch-clamp literature data. Assay […]
Phagocytosis assay
Phagocytosis assay Phagocytosis is a key immune mechanism involves uptake of solid particles into phagosomes, and clearing phagocytosed particles (e.g., pathogens, aggregates of macromolecules, and apoptotic cells). Here, we measured Phagocytosis in whole blood, iPSC Microgila and RAW cells by quantifying engulfed pHrodo bacterial particles. Phagocytosis in human whole blood Figure-A: Healthy donor whole blood […]
Neutrophil Chemotaxis assay
Neutrophil Chemotaxis Assay A robust In vitro assay developed using fresh Neutrophils isolated from healthy donor whole blood. The assay was used to measure compound potency on Neutrophil migration. The assay utilises Transwell® Permeable Support systems. Neutrophil Chemotaxis activation Figure-A: Fold increase in Neutrophil migration over Vehicle response upon addition of FBS (0% to 20%). […]
Mitochondrial toxicity assessment
Mitochondrial toxicity assay services for safety assessment at an early stage Mitochondrial toxicity is a major contributor to drug-induced adverse effects and is an important factor in the failure of drug candidates during development. Damage to mitochondrial function can disrupt cellular energy production, leading to cell dysfunction or death. At BioMedha, we provide mitochondrial toxicity […]
Macrophage assays
Macrophage assays Isolated monocytes from fresh healthy donor whole blood were differentiated into macrophages. Validated by cytokine profiling and surface marker expression. Suitable In vitro assay models for measuring compound effect on macrophage cell function and gene expression. Macrophage differentiation Figure-A: Methods for isolation of monocytes, maintenance, and differentiation into M1 and M2 phenotype macrophages. […]
Target expression studies
Different approaches measuring target expression Target expression patterns provide valuable insight in target validation, understanding biology, and the molecular basis for compound effect. Expression studies can be performed at protein level, mRNA level, secretion level, at surface level, and expression by immunofluorescence. Gene expression by qPCR (RT-PCR) Figure-A: CD44, CXCL16, IL-1b and CD123 mRNA amplification […]
Dendritic cell assays
Dendritic cell assays Isolated monocytes from fresh healthy donor whole blood were differentiated into dendritic cells, validated by cytokine profiling and surface marker expression. Suitable in vitro assay models for measuring a compound’s effect on dendritic cell function and gene expression. Dendritic cell differentiation Figure-A: Methods for isolation of monocytes, maintenance, and differentiation into dendritic […]
Immunogenicity assessment
Cytokine storm assay services for immunogenicity risk assessment Cytokine storm assays are widely implemented to assess immunogenicity risk at an early stage. Uncontrolled cytokine release activation can lead to severe inflammatory responses and has been associated with serious adverse events during clinical development. At BioMedha, we perform cytokine storm assays using human donor PBMCs to […]
Biomarker analysis
Biomarker assays: analysis of ex-vivo samples Ex-vivo sample analysis provides a means to confirm the in-vivo effect of test compounds and to identify the correlation between in vivo efficacy and biomarkers. In this study, ex-vivo rat and mouse plasma samples, and human plasma from healthy donors were analysed for cytokines as biomarkers. Rat plasma (ex-vivo) […]